Composite

Part:BBa_K3182000:Design

Designed by: Oliver Hild Walett   Group: iGEM19_Linkoping_Sweden   (2019-07-08)


pT7-CBDcipA-AsPink


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 580
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Removed one EcoRI site inside the cellulose binding domain. Also added a BamHI site on the end of the thrombin site to enable the ability to change the fusion protein. BamHI was chosen because of the recognition sequence codes for one glycine and one serine, which is also the end recognition of the thrombin site. These amino acids also have very small influence on the fusion protein.


Source

Cellulose binding domain originates from Clostridium thermocellum. AsPink originates from Anemonia sulcata. The GS-linker with the thrombin site is of our own design.

References